hace2 specific goat antibody  (R&D Systems)

Journal: bioRxiv

Article Title: The Mac1 ADP-ribosylhydrolase is a Therapeutic Target for SARS-CoV-2

doi: 10.1101/2024.08.08.606661

Figure Lengend Snippet: (A) K18-hACE2 mice were infected with SARS-CoV-2 WA1 strain and dosed with nirmatrelvir (300 mg/kg) or vehicle BID. Each group was composed of n=5 mice. The percent weight loss is presented as mean ± SD. (B) Survival curve in vehicle or nirmatrelvir treated mice based on the weight loss humane endpoint.

Article Snippet: A549-ACE2h cells were generated by stably expressing hACE2 ( ) and further selecting for high ACE2 expression levels via FACS with Alexa Fluor® 647 conjugated to a hACE2-specific antibody (FAB9332R, R&D systems).

Techniques: Infection

Journal: bioRxiv

Article Title: The Mac1 ADP-ribosylhydrolase is a Therapeutic Target for SARS-CoV-2

doi: 10.1101/2024.08.08.606661

Figure Lengend Snippet: (A) K18-hACE2 mice were intranasally infected and intraperitoneally dosed as indicated with either vehicle (n=10) or AVI-4206 (n=15). Mice infected with WA1 N40D mutant, which lacks Mac1 catalytic activity, served as a positive control (n=10). Lungs were harvested at indicated time points for virus titration by plaque assay. (B) The percent body weight loss for all animals. The data are presented as mean ± SD. (C) Survival curve plotted based on the percent weight loss humane endpoint. (D) Viral load in the lungs and brain of infected mice at the indicated time points. The data are shown as mean ± s.e.m. *, P < 0.05; **, P < 0.01 by Mann Whitney’s test relative to the vehicle control. (E) Schematics and graphs demonstrating the abundance of indicated cytokines at 4 and 7 days post-infection in the lungs of infected mice. The data are presented as mean ± s.e.m. *, P < 0.05; **, P < 0.01 by two-tailed Student’s t-test relative to the vehicle control at each timepoint. None of the mice reached the humane endpoint at day 4 post-infection. For mice that reached the humane endpoint before day 7 post-infection, the tissues were collected and analyzed with mice at the 7 day time point.

Article Snippet: A549-ACE2h cells were generated by stably expressing hACE2 ( ) and further selecting for high ACE2 expression levels via FACS with Alexa Fluor® 647 conjugated to a hACE2-specific antibody (FAB9332R, R&D systems).

Techniques: Infection, Mutagenesis, Activity Assay, Positive Control, Virus, Titration, Plaque Assay, Control, Two Tailed Test

Journal: bioRxiv

Article Title: The Mac1 ADP-ribosylhydrolase is a Therapeutic Target for SARS-CoV-2

doi: 10.1101/2024.08.08.606661

Figure Lengend Snippet: (A) K18-hACE2 mice were intranasally infected SARS-CoV-2 WA1 or SARS-CoV-2 WA1 Mac1 N40D mutant. Mice were treated as indicated with AVI-4206 (BID, 30 mg/kg) or vehicle. Each group was composed of n=10 mice (5 mice per time point). (B) The percent body weight loss is presented as mean ± SD. (C) Survival curve based on the percent body weight loss humane endpoint. (D) Viral load in the lung at indicated time points is presented as mean ± s.e.m. **, P < 0.01 by Mann Whitney’s test relative to the vehicle control.

Article Snippet: A549-ACE2h cells were generated by stably expressing hACE2 ( ) and further selecting for high ACE2 expression levels via FACS with Alexa Fluor® 647 conjugated to a hACE2-specific antibody (FAB9332R, R&D systems).

Techniques: Infection, Mutagenesis, Control

Journal: International Journal of Biological Macromolecules

Article Title: A SARS-CoV-2 vaccine based on conjugation of SARS-CoV-2 RBD with IC28 peptide and mannan

doi: 10.1016/j.ijbiomac.2022.09.180

Figure Lengend Snippet: SPR analysis of the interaction between RBD and hACE2 in the presence of the diluted mice sera. The responses before (a) and after normalization of the initial response to zero (b) were recorded during the incubation time of 300 s. The mixture of RBD and hACE2 was in the presence of PBS buffer (1), 8000- (2), 4000- (3), 2000- (4), 1000- (5), 500- (6), 250- (7), and 125-fold (8) diluted sera of the RBD-IC28-M group, respectively.

Article Snippet: Human angiotensin-converting enzyme 2 (hACE2), hACE2-specific rabbit antibody, and HRP-conjugated goat antirabbit IgG antibody were ordered from Sino Biological (Beijing, China).

Techniques: Incubation